LIQUICELIN-E 3ml tulip
₹795.00
Tags:
| Packaging Type | Box |
| Usage/Application | For Partial Thromboplastin Time (Aptt) Determination Using Ellagic Acid, As An Activator |
| Packaging Size | 12 x 3ml |
| Certification | CE |
| Shelf Life | Once opened the reagent is stable for 3 months at 2-8 Degree Celsius |
| Storage Temp. | Store the reagent at 2-8 Degree Celsius.Do not Freeze |
| Manufacturer | Tulip |
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Product Details
Summary
The arrest of bleeding depends upon primary platelet plug formed along with the formation of a stable fibrin clot. Formation of this clot involves the sequential interaction of a series of plasma proteins in a highly ordered and complex manner and also the interaction of these complexes with blood platelets and materials released from the tissues. Activated Partial Thromboplastin Time is prolonged by a deficiency of coagulation factors of the intrinsic pathway of the human coagulation mechanism such as factor XII, XI, IX, VIII, X, V, II and Fibrinogen. Determination of APTT helps in estimating abnormality in most of the clotting factors of the intrinsic pathway including congenital deficiency of factor VIII, IX, XI and XII and is also a sensitive procedure for generating heparin response curves for monitoring heparin therapy.
Principle
Cephaloplastin activates the coagulation factors of the intrinsic pathway of the coagulation mechanism in the presence of calcium ions. APTT is prolonged by a deficiency of one or more of these clotting factors of the intrinsic pathway and in the presence of coagulation inhibitors like heparin.
Sample collection and preparation
No special preparation of the patient is required prior to sample collection by approved techniques. Withdraw blood without undue venous stasis and without frothing into a plastic syringe fitted with a short needle of 19 to 20 SWG. The veinpuncture must be a 'clean' one and, if there is any difficulty, take a new syringe and needle and try another vein. Transfer the blood into tubes, after detaching the needle from the syringe. Mix exactly nine parts of freshly collected blood with one part of tri-sodium citrate (0.11 mol/l, 3.2%). Centrifuge immediately for 15 minutes at 1500 g and transfer the plasma into a clean test tube. Plasma must be tested within three hours of blood collection. For heparin determination, platelet deficient plasma should be used, hence higher centrifugation time is required.
The arrest of bleeding depends upon primary platelet plug formed along with the formation of a stable fibrin clot. Formation of this clot involves the sequential interaction of a series of plasma proteins in a highly ordered and complex manner and also the interaction of these complexes with blood platelets and materials released from the tissues. Activated Partial Thromboplastin Time is prolonged by a deficiency of coagulation factors of the intrinsic pathway of the human coagulation mechanism such as factor XII, XI, IX, VIII, X, V, II and Fibrinogen. Determination of APTT helps in estimating abnormality in most of the clotting factors of the intrinsic pathway including congenital deficiency of factor VIII, IX, XI and XII and is also a sensitive procedure for generating heparin response curves for monitoring heparin therapy.
Principle
Cephaloplastin activates the coagulation factors of the intrinsic pathway of the coagulation mechanism in the presence of calcium ions. APTT is prolonged by a deficiency of one or more of these clotting factors of the intrinsic pathway and in the presence of coagulation inhibitors like heparin.
Sample collection and preparation
No special preparation of the patient is required prior to sample collection by approved techniques. Withdraw blood without undue venous stasis and without frothing into a plastic syringe fitted with a short needle of 19 to 20 SWG. The veinpuncture must be a 'clean' one and, if there is any difficulty, take a new syringe and needle and try another vein. Transfer the blood into tubes, after detaching the needle from the syringe. Mix exactly nine parts of freshly collected blood with one part of tri-sodium citrate (0.11 mol/l, 3.2%). Centrifuge immediately for 15 minutes at 1500 g and transfer the plasma into a clean test tube. Plasma must be tested within three hours of blood collection. For heparin determination, platelet deficient plasma should be used, hence higher centrifugation time is required.
